Methods in Enzymology G protein coupled receptors : structure / edited by P. Michael Conn

Expression of GPCRs in Pichia pastoris for structural studies -- Conformational ensemble view of G protein-couples receptors and the effect of mutations and ligand binding -- Structural evolution of G-protein-coupled receptors: a sequence space approach -- Directed evolution of G-protein-coupled receptors for high functional expression and detergent stability -- The role of hydrophobic amino acids in the structure and function of the Rhodopsin family of G protein-coupled receptors -- Structure of [beta]-adrenergic receptors - Advances in methods to characterize ligand-induced ionic lock and Rotamer toggle molecular switch in G protein-coupled receptors -- Crystallogenesis of adenosine A2A receptor-T4 lysozyme fusion protein: a practical route for the structure -- Probing GPCR structure: adenosine and P2Y nucleotide receptors -- Strategies for studying the ligand binding site of GPCRs: photoaffinity labeling of the VPAC1 receptor, a prototype of class B GPCRs -- Expression of mammalian G protein-coupled receptors in Caenorhabditis elegans -- Expression, purification, and structural analysis of intracellular C-termini from metabotropic glutamate receptors -- Unnatural amino acid mutagenesis of GPCRs using amber codon suppression and bioorthogonal labeling -- Mapping a ligand binding site using genetically encoded photoactivatable crosslinkers -- Alternative mRNA splicing of G protein-coupled receptors -- Functional residues essential for the activation of the C1 Cannabinoid receptor